The invention features methods and compositions for screening for agents
that modulate replication of a virus, particularly Flaviviridae virus
(particularly hepatitis C virus (HCV)), where the methods provide for
detection of agents that modulate the binding of TBC and NS5A, the
inhibition of TBC activity, inhibition of Rab1 activity, and/or the
expression of the TBC protein and/or Rab1 protein. The invention also
features methods of controlling viral replication, and agents useful in
such methods.