The present invention relates to a method for preparing 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof by culturing organisms, in particular yeasts. Furthermore, the invention relates to the preparation of the nucleic acid constructs required for preparing the genetically modified organisms and to said genetically modified organisms, in particular yeasts, themselves.

 
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> Methods for protein expression in mammalian cells in serum-free medium

> Sequence-determined DNA fragments encoding sterol desaturase proteins

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