Disclosed are compositions and methods for increasing the longevity of a
cell culture and permitting the increased production of proteins,
preferably recombinant proteins, such as antibodies, peptides, enzymes,
growth factors, interleukins, interferons, hormones, and vaccines. Cells
transfected with an apoptosis-inhibiting gene or vector, such as a triple
mutant Bcl-2 gene, can survive longer in culture, resulting in extension
of the state and yield of protein biosynthesis. Such transfected cells
exhibit maximal cell densities that equal or exceed the maximal density
achieved by the parent cell lines. Transfected cells can also be
pre-adapted for growth in serum-free medium, greatly decreasing the time
required to obtain protein production in serum-free medium. In certain
methods, the pre-adapted cells can be used for protein production
following transfection under serum-free conditions. In preferred
embodiments, the cells of use are SpESF or SpESF-X cells.