A separating agent which combines a high optical resolving power inherent
in polysaccharide derivatives with sufficient solvent resistance and a
method with which the separating agent can be produced efficiently in
short steps. The separating agent for enantiomeric isomers is
characterized in that a polymerizable polysaccharide derivative of a
polysaccharide derivative having polymerizable functional groups and a
polymerizable monomer having polymerizable unsaturated groups are
copolymerized with a carrier having polymerizable functional groups to be
chemically bound mutually. The separating agent is preferably used for
high performance liquid chromatography (HPLC) and has an excellent
solvent resistance.