The present invention provides a method for selective and high-yield separation,
concentration, and recovery of desired cells from a blood sample. The method of
the present invention is characterized in that the blood sample is caused to interact
with lectins under conditions in which the cell membranes are inactive and cell-lectin
complexes/non-complexes are formed, the sample containing these cell-lectin complexes/non-complexes
is incubated together with a substrate, the surface of which is covered with polymers
having carbohydrate chains which are specifically recognized by the lectins, and
the cells are immobilized on the surface of the substrate via the lectins, and
subsequently, the liquid layer and the solid phase are separated, and the desired
blood cells are recovered from the liquid phase and/or the solid phase, and these
lectins are in such an amount that although the cells to be recovered from the
solid phase bind to the solid phase with the polymer, the cells to be recovered
from the liquid phase do not bind to the solid phase with the polymer.
