A harmonic generation microscopy employs a laser device that emits a laser beam
having a predetermined wavelength that causes no autofluorescence in a biological
sample and that, after excited, induces both the second and third harmonic waves.
The laser beam is projected onto a sample and an observation beam from the sample
is received. The observation beam is directed through a splitter to separate the
second harmonic wave and the third harmonic wave both of which are then converted
into corresponding electrical signals. The electrical signals are fed to a computer-based
image processing equipment to form an image of the sample on the basis of the second
and third harmonic waves.
