A method for isolating and purifying nucleic acids with an improved recovery
yield
is provided. A mixed solution containing the nucleic acids, salts, and an organic
solvent is contacted with an adsorption support to cause the nucleic acids to be
adsorbed on the support. Then, the nucleic acids are desorbed from the support
using an elution buffer. At least one compound containing 2 to 10 carbon atoms
as selected from the group consisting of aliphatic ether, aliphatic ester, and
aliphatic ketone is used as the organic solvent. The method improves the yield
of nucleic acids collection, is easy to implement and less susceptible to contamination.
