Homo-doubly labeled compositions for the detection of enzyme activity in biological samples

   
   

The present invention provides for novel reagents whose fluorescence changes upon cleavage or a change in conformation of a backbone. The reagents comprise a backbone (e.g. nucleic acid, polypeptide, etc.) joining two fluorophores of the same species whereby the fluorophores form an H-dimer resulting in quenching of the fluorescence of the fluorophores. When the backbone is cleaved or changes conformation, the fluorophores are separated, no longer forming an H-type dimer, and are de-quenched thereby providing a detectable signal. The use of a single fluorophore rather than an "acceptor-donor" fluoresecence resonance energy transfer system offers synthesis and performance advantages.

 
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< Polymer-protein surfactants

< Polynucleotides encoding aminolevulinic acid biosynthetic enzymes

> Interleukin-2 mutein expressed from mammalian cells

> Nucleic acid molecules encoding hyperactive mutant phytochromes and uses thereof

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