Methods useful for improving results obtained with enzyme-based polynucleotide
amplification reactions. More particularly, the invented methods are useful for:
(1) promoting amplification of template-specific products such that the amount
of amplicon produced reflects the pre-amplification amount of analyte, even in
reactions primed with low levels of analyte polynucleotide; (2) facilitating biological
specimen processing such that the amount of amplicon produced in subsequent amplification
reactions will be substantially independent of the efficiency of analyte polynucleotide
isolation from the specimen; and (3) controlling the amount of analyte amplicon
produced in the amplification reaction.
