The inventive method of producing a eukaryotic viral vector comprises contacting
a eukaryotic cell, which comprises a unique enzyme that nicks or cleaves a DNA
molecule, with a recombinant phage vector, or contacting a eukaryotic cell, which
does not comprise a unique enzyme that nicks or cleaves a DNA molecule, simultaneously
or sequentially, in either order, with (i) a unique enzyme that nicks or cleaves
a DNA molecule, and (ii) a recombinant phage vector. The recombinant phage vector
comprises the DNA molecule comprising (a) a eukaryotic viral vector genome comprising
a coding sequence, (b) a phage packaging site that is not contained within the
eukaryotic viral vector genome, and (c) a promoter that is operably linked to the
coding sequence.
Alternatively, the DNA molecule is not present within the recombinant
phage vector. The eukaryotic cell is contacted with the first DNA molecule and
a recombinant phage vector. The first DNA molecule comprises a replication deficient
eukaryotic viral vector genome comprising at least one adenoviral inverted terminal
repeat and a packaging signal. The recombinant phage vector comprises a second
DNA molecule and a phage packaging site, wherein the second DNA molecule complements
in trans the replication deficient eukaryotic viral vector genome.
The DNA molecule(s) enter the eukaryotic cell, and the unique enzyme nicks or
cleaves the DNA molecule in the eukaryotic cell in at least one region not contained
within the eukaryotic viral vector genome, thereby inducing the production of and
ultimately producing a eukaryotic viral vector.
