A fundus camera is provided with a focus aid mark projection system (5, 9, 10, 11, 12, 13) that includes a focus aid mark focussing lens (10) and other optical components such that the focus aid mark focussing lens (10) is attached to the focussing lens (6) of the imaging system and moves with that lens, thereby maintaining the focal planes of the focus aid mark projection system and the imaging system co-planar. An associated alignment mark projection system (35, 34, 33, 32) includes optics that utilize part of the existing fundus camera illumination system (35) to project a plurality of alignment marks onto the iris of the subject eye.