A system and method of determining locations of one or more RFID tags within an RFID environment. The system includes a plurality of RFID readers, each operative to transmit and receive RF signals for scanning a tag disposed within an RF coverage region associated with the reader, and for receiving tag data in response to the scanning of the tag. A plurality of sub-locations is determined within the environment, each corresponding to at least a portion of at least one of a plurality of RF coverage regions associated with the readers. The sub-locations are mapped to a plurality of predefined locations within the environment. A reader scans a tag, and receives tag scan data from the tag in response to the scanning of the tag. The tag scan data includes a tag identifier associated with the scanned tag. The tag scan data is mapped to the sub-locations based on the RF coverage region associated with the reader. The location of the scanned tag is then determined with reference to the predefined locations within the environment, based on the tag identifier included in the tag scan data, the mapping of the tag scan data to the sub-locations, and the mapping of the sub-locations to the predefined locations.