The present invention relates to a novel tobacco cultivar designated AOB 171, which has low to intermediate nicotine content. The invention provides seeds of the cultivar AOB 171, plants and parts thereof of the cultivar AOB 171, a tissue culture derived from the cultivar AOB 171, hybrids produced from cultivar AOB 171 and lines derived from cultivar AOB 171, as well as genetically modified forms of the foregoing plants and tissue culture. Also provided are methods of producing cultivar AOB 171 plants, cultivar AOB 171 hybrid plants, and tobacco lines derived from cultivar AOB 171. In addition, products produced from the plants of the present invention are provided.