A portable tomosynthesis imaging system is disclosed that includes a portable X-ray source assembly and a portable detector assembly. The source assembly may be coupled to a portable power supply and controller such that multiple projection X-ray images may be obtained at a site that is not accessible by conventional tomosynthesis imaging systems. Image data may be transmitted from the detector by wired or wireless communication. Tomosynthesis image reconstruction may be performed locally at the portable system or remotely by the transmission of raw or filtered image data from the portable system. The portable system is particularly well-suited to field deployment, such as at accident scenes, scenes of natural or other disasters, or in confined clinical settings.