An extract is derived from Pueraria candollei var. mirifica A Shaw. & Suvat. for topical and internal use in treating a subject. The extract is obtained by a sequential extraction process using a sequence of different solvents or mixtures of solvents in varying ratios and thereafter combining the liquid extracts. The liquid extract is then evaporated to dryness to obtain the extract. The extract is used in a topical composition that when applied to the skin reduces wrinkles without eye irritation.