According to the invention, there is provided a novel soybean variety designated XB53S08. This invention thus relates to the seeds of soybean variety XB53S08, to the plants of soybean XB53S08 to plant parts of soybean variety XB53S08 and to methods for producing a soybean plant produced by crossing plants of the soybean variety XB53S08 with another soybean plant, using XB53S08 as either the male or the female parent.