Methods for making cDNA molecules, for amplification of RNA by PCR and for
preparation of cDNA libraries are provided. Kits for making cDNA
molecules also are provided. Compositions are also provided comprising
mixtures of reagents, including reverse transcriptases, buffers,
cofactors and other components, suitable for immediate use in conversion
of RNA into cDNA and RT PCR without dilution or addition of further
components. These compositions are useful, alone or in the form of kits,
for cDNA synthesis or nucleic acid amplification (e.g., by the Polymerase
Chain Reaction) or for any procedure utilizing reverse transcriptases in
a variety of research, medical, diagnostic, forensic and agricultural
applications.