A targeting vector was constructed by replacing exon regions in the SGRF
gene with appropriate drug marker genes. This vector was transfected into
mouse ES cell lines to obtain chimeric mice, which were then crossed with
C57BL/6J mice to obtain mice comprising cells in which one SGRF gene
alleles was inactivated. By crossing these mice with each other, the
present inventors succeeded in producing mice in which both SGRF gene
alleles were inactivated. These genetically modified animals can be used
to predict the side effects of drugs such as SGRF antagonists.