The invention relates to a computer implemented method for processing of
microscopic images to detect objects of interest. The method includes
subjecting the microscopic image to a bandpass filtering to obtain a
filtered image, wherein the bandpass filtering is such as to suppress the
noise and any objects which are larger than a predetermined size; and
processing the filtered image at a plurality of progressively decreasing
threshold levels. The processing at each threshold level includes
detecting the objects of interest using an object labelling algorithm and
removing the detected objects detected at a given threshold level from
the working image before proceeding to the next threshold level.