A process of detection of the causative agent of Johne's disease (Mycobacterium avium subsp. paratuberculosis) (MAP) by detecting shedding of surface protein of MAP. A preferred way is use of surface enhanced Raman Spectroscopy. The system of detecting MAP shedding of protein provides early detection and diagnosis, and therefore allows early treatment for Johne's disease in ruminant animals.

 
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