The invention provides seed and plants of the lettuce line designated P2428-5006090. The invention thus relates to the plants, seeds and tissue cultures of lettuce line P2428-5006090, and to methods for producing a lettuce plant produced by crossing a plant of lettuce line P2428-5006090 with itself or with another lettuce plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of lettuce line P2428-5006090, including the gametes of such plants.