An imaging system comprises a light source module configured to generate a combination beam, a controller configured to control the light source module, an image-capturing module configured to capture the reflected beam by a sample. The light source module comprises a plurality of light-emitting devices configured to emit lights of different wavelengths, and the controller is configured to drive the light-emitting devices to emit the lights to form the combination beam consisting of at least two lights of different wavelengths. An imaging method comprises the steps of forming a first combination beam consisting essentially of at least two lights of different wavelengths, capturing the reflected first combination beam by a sample to have a first image, forming a second combination beam consisting of at least two lights of different wavelengths and capturing the reflected second combination beam by the sample to have a second image.