The invention provides a method for generating a T cell comprising a T cell receptor capable of specifically binding an antigen of interest, comprising: --providing a hematopoietic stem cell and/or a precursor cell of a T cell with a nucleic acid sequence comprising at least part of a rearranged gene encoding a TCR chain, or a functional equivalent thereof; and--allowing for differentiation of said stem cell and/or precursor cell and generation of at least one T cell derived from said stem cell and/or precursor cell.