This invention provides: 1) methods for the identification of broad spectrum holins with a high level of nonenzymatic activity in membranes; 2) conditions required for maintaining and increasing the anti-microbial and anti-pest efficacy of holins in gene fusions; 3) a method for effective targeting of holins expressed in plants through use of leader peptide to direct the holin protein in the plant apoplast and xylem; 4) methods for the control of bacterial and fungal diseases of plants and control of insect and nematode pests that attack plants by expression of gene fusion involving holins, C-terminal additions and leader peptides, and optionally, endolysis; 5) methods for increasing the shelf of cut flowers, and 6) transgenic plants useful for the production of novel antimicrobial proteins based upon holins.

 
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~ 00531