Provided are methods for detecting expression of nucleic acids encoding mutant fluorescent proteins. In the methods, a nucleic acid encoding a mutant fluorescent protein, or a full complement thereof, is introduced into a cell or organism. The cell or organism is allowed to replicate. Expression of the nucleic acid is detected by emission of fluorescent light. Animals including a nucleic acid encoding a mutant fluorescent protein, or a full complement thereof, are also provided.

 
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