Provided are methods for detecting a target nucleic acid in a biological
sample using RNA amplification. In an embodiment, provided is a method
that comprises (a) amplifying a target nucleic acid, or portion thereof,
using a primer comprising a sequence that is complementary to a
polynucleotide sequence in the target nucleic acid, or a complement
thereof, and a sequence that encodes an RNA polymerase promoter; (b)
synthesizing RNA using an RNA polymerase that recognizes the promoter;
and (c) detecting the presence or absence of the resulting RNA using mass
spectrometry thereby detecting the presence or absence of the target
nucleic acid in the biological sample.