A method of calibrating a monolithic transmitter photonic integrated circuit (TxPIC) chip is disclosed where the chip contains integrated arrays of laser sources and electro-optic modulators forming a plurality of different wavelength signal channels where each laser source on the chip is sequentially selected and tested for the output power and operational wavelength. Calibration data is initially determined by checking an amount of output power of each laser output and any offset of each laser operational wavelength from a desired predetermined value. Then, adjustment of the operational wavelength of each laser source is accomplished to substantially match the desired predetermined value. The laser source output power and operational wavelength may then be rechecked to determine if there is any remaining offset of each laser operational wavelength from the desired predetermined value. If so, the laser source output power can be readjusted to a desired amount and its operational wavelength can be readjusted to the desired predetermined wavelength value. The final calibration data it then stored for future reference as a benchmark in later adjustment of laser source output power and operational wavelength for each laser source on the tested chip when the chip has been installed in the field.