For give a direct methanol fuel cell which secures the fuel supply to the catalyst layer, reliably discharge generated carbon dioxide gas, and has excellent electricity generation ability, at least a portion of the anode side flow path is divided by a film having water-repellency and gas permeability to a first flow path portion positioned at the membrane electrode assembly side and a second flow path portion positioned at the bottom side of the anode side flow path where a fuel mainly flows in the first flow path portion and carbon dioxide mainly flows in the second flow path portion.