A device for conducting an assay to determine the presence or amount of an analyte in a fluid sample. The device includes a flow path that facilitates fluidic flow by capillary action. The flow path has a first fluid permeable region, a second fluid permeable region, a barrier that inhibits fluidic flow between the first and second regions, and a fluidic bridge. The bridge includes a fluid permeable material overlapping at least a portion of each of the first region, the barrier and the second region.