A non-continuous immunoassay device which includes two or more separated
pads for immunoassay analysis, and is capable of controlling the
migration speed of a mobile phase between the separated pads, and an
immunoassay method using the same are disclosed. The immunoassay device
includes a first pad receiving a mobile phase; a second pad which is
spatially separated from the first pad by a predetermined distance, and
to which the mobile phase migrates; an upper case for covering the upper
parts of the first pad and the second pad; a lower case for covering the
lower parts of the first pad and the second pad; and a connecting member
which is formed on at least one of the upper case and the lower case, and
located between the first pad and the second pad to form a passage for
moving the liquid sample.