To provide a light-emitting device in which a partition wall portion and a second electrode are not peeled off and display failure hardly occurs, a method of manufacturing the light-emitting device, and an electronic apparatus comprising the light-emitting device. An area in which a lyophobic portion is not formed is formed on a partition wall other than peripheries of pixel electrodes. In addition, a negative electrode is formed to directly come in contact with the area of the partition wall in which the lyophobic portion is not formed.