The present invention provides novel compositions comprising a Nigella sativa L. lipid fraction. The Nigella sativa L. lipid fraction is an oily fraction primarily comprised of long chain fatty acids, volatile oils and sterols. The present invention also provides novel methods using compositions comprising the Nigella sativa L. lipid fraction for treating and preventing skin infections, wounds, bacterial infections, respiratory diseases and disorders, cellulite, cardiovascular disorders, septic infections, and the like.