Membrane holder for membrane adsorber chromatography comprising a housing body (12) with a reception space for at least one layer of adsorber membranes (22) and at least one first housing cap (34) for closing a first reception space opening, which can be fixed to the housing body (12), a liquid stream being introducible via first liquid guide means (36) through the first housing cap (34) and the first reception opening into the reception opening, so that it passes through the adsorber membranes (22) and flows away through a second reception space opening. The first housing cap (34) can be screwed as a first union nut onto the housing body (12) and a first brace element (24), which can be clamped between the first cap (34) and the housing body (12) via a flange (32) extending beyond the first reception space opening, can be fixed leaktightly in a basket-like insert (14) with a liquid-tight wall and a perforated bottom (18), which is used to receive the adsorber membranes (22). The outer contour of the basket-like insert (14) being fitted in a leaktight fashion onto an inner contour of the reception space.