A fluid container and method for inhibiting the growth of microbes in liquid nutrient in a container, the container having an interior surface having a metal-ion sequestering agent and/or antimicrobial agent provided in a localized area of the container formed by a two shot molding, insert molding or two shot injection stretch blow molding process, said metal ion sequestering agent provided for the removing and/or inhibiting growth of microbes in the liquid nutrient.