An absorbent article, such as a feminine care product, includes a liquid permeable top cover, a generally liquid impermeable outer cover, and an absorbent structure disposed between the top cover and outer cover. A band of spaced apart deposits of a barrier substance material, such as a phase-change liquid, are defined on the top cover (inner or outer surface) along a portion of opposite lateral sides of the article. The deposits have a desired height of topography with respect to a surface of the top cover. The deposits are defined such that areas of the top cover are exposed within the band, the band thereby being permeable to bodily fluids insulting the article within the band or from above the band while acting as a fluid barrier against lateral flow of bodily fluids across the band.

 
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> In situ growth, freezing and testing of cultured cells

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