The Diaphanousrelated formins (DRFs) constitute a subclass of FH proteins
known for their ability to bind activated Rho subfamily of small
GTP-binding proteins which link FH proteins to cellular signalling
pathways. The present inventor has identified a new homology domain
unique to the DRFs that termed the DRF-autoregulatory domain (DAD) in the
extreme Cterminus. DAD is involved in intramolecular binding with the
GTPase binding domain (GBD) of the DRFs. Disclosed herein are
compositions and methods that can disrupt this binding, and cause changes
in cells including actin polymerization and/or stabilization of actin
fibers resulting in growth inhibition or cell death by apoptosis. A
preferred composition is peptide or polypeptide of no more than about 130
amino acids comprising the DAD peptide having the amino acid sequence
[GA]-[VA]-M-D-x-L-L-E-x-L-[KRQ]-x-[GA]-[SGA]-[AP] (wherein amino acids
within a set of brackets are interchangeable and x means any amino acid).
Fusion proteins comprising DAD are also disclosed as are nucleic acids
and expression vectors encoding these peptides and proteins.