Genetic vaccine which comprises plasmid(s) containing genes coding for
antigens of enterotoxigenic Escherichia coli (ETEC) strains is disclosed.
Additionally, plasmids may consist of multiple copies of the same antigen
(i.e. K88 or K99 fimbrial antigen) or multiple antigens (ie. K88 and K99
fimbrial antigens) and genetic adjuvants such as cytokines (IL-2, IL-4 &
GM-CSF), costimulatory molecules (CD80 & CD86) or chemokines or
immunostimulatory sequences. A method for isolating antibodies from
chicken egg yolk for passive immunization of animals, as well as humans
to control diarrhoeal diseases using the genetic vaccines is also
disclosed.