It is an object of the present invention to provide means for efficiently
obtaining peptides that exhibit a high affinity toward a given target.
Peptides are designed by the steps of (1) performing an affinity assay
using a plurality of peptides having different peptide sequences and a
target to obtain affinity data for each of the peptide sequences toward
the target; (2) selecting high-affinity peptide sequences and
low-affinity peptide sequences; (3) digitizing predetermined
property(ies) of amino acids for each location from N-terminal or
C-terminal to transform each of the selected peptide sequences into
numerical data; (4) performing Fuzzy Neural Network analysis by using the
obtained numerical data as input variables to construct a prediction
model; (5) extracting from the constructed prediction model one or two or
more rules wherein the amino acids and the properties are related to each
other on one or more locations on the sequences, said rules representing
the characteristics of high-affinity peptide sequence; and (6) designing
a peptide according to the extracted rules.