The present invention relates to a high-speed high-temperature nematic liquid crystal composition and a liquid crystal display comprising the same, particularly to a high-speed high-temperature nematic liquid crystal composition which comprises a nematic liquid crystal compound having an isothionate group on the end aromatic ring and having one or more kinds of fluorine atoms, and thus satisfies a high response speed property and has a high phase transition temperature of liquid crystal, a low operating voltage, and a broad nematic phase temperature range, and a liquid crystal cell comprising the same, and a liquid crystal display using the same.