A method for assigning a single frequency sub-channel to a plurality of MSs (Mobile Stations) in an OFDM (Orthogonal Frequency Division Multiplexing) system utilizing SDM (Spatial Division Multiplexing) with multiple transmit/receive antennas. In the method, a BS (Base Station) can effectively assign a pilot channel to estimate a downlink channel. The BS divides the single frequency sub-channel into a plurality of spatial channels, and sequentially assigns the plurality of spatial channels to the MSs having the maximum communication capacity, thereby transmitting signals of the MSs through the single frequency sub-channel.