The present invention provides mutant D-aminoacylases and use thereof. The
mutant D-aminoacylases are hard to be inhibited by the substrate and,
comprise the amino acid sequences of the D-aminoacylase derived from
Alcaligenes denitrificans subsp. xylosoxydans MI-4 strain, wherein amino
acid residues at specific sites have been modified. The mutants of the
present invention have high reaction specificity as well as resistance to
inhibition by the substrate. The present invention enables high-yield
production of D-amino acids using higher concentrations of
N-acyl-DL-amino acid as the substrate. The mutants of the present
invention are useful in producing D-tryptophan in particular.