Methods for producing ligand arrays, e.g., peptide and nucleic acid
arrays, as well as the arrays produced thereby, methods for use of the
arrays and kits that include the same are provided. In one embodiment of
the subject methods, a substrate having a surface displaying acetate
functional groups that produce surface bound hydroxyl functional groups
upon hydrolysis is first provided, where the acetate groups are then
hydrolyzed to hydroxyl functional groups. In another embodiment of the
subject methods, a substrate having a surface displaying alkenyl
functional groups is first provided, where the alkenyl functional groups
are then converted to carboxylic functional groups. The resultant
substrates, optionally after an additional functionalization step, are
then contacted with ligands, e.g., via deposition of each different
ligand onto a different region of the surface, resulting in covalent
attachment of the contacted ligands to the surface. The subject methods
find use in the preparation of a variety of different types of arrays,
where the produced arrays find use in a variety of different
applications, including both genomic and proteomic applications.