Provided is a method of culturing a successor cell from a precursor cell
comprising co-culturing a precursor cell such as, without limitation, a
human embryonic stem cell or a neuronal stem cell with an adult
mesenchymal stem cell. In one embodiment, the adult mesenchymal stem cell
is derived from adipose tissue. Also provided is a composition comprising
mesenchymal stem cells in a growth matrix biologically-compatible with
the cells. In another embodiment, a method of regenerating tissue is
provided comprising introducing into a patient a cell growth niche
comprising either or both of mesenchymal stem cells and neuronal stem
cell or successor cell in a growth matrix biologically-compatible with
the cells. In another embodiment, a method of regenerating tissue is
provided comprising introducing into a patient a cell growth niche
comprising matrix, cells and slow release polymer beads containing growth
factors.