The instant invention provides methods, reagents, and computational tools
for designing non-natural substrate analogs for enzymes, especially for
designing unnatural amino acid analogs for aminoacyl tRNA Synthetases
(AARSs), such as the Phe tRNA Synthetase. The instant invention also
provides methods to incorporate unnatural amino acid analogs, especially
those with interesting functional groups, into protein products to
generate proteins of modified or novel functions.