The present invention includes methods for integrating a modifying polynucleotide into a target polynucleotide in a cell, or instance, a fish cell or a mouse cell, and methods for making chimeric animals, heterozygous animals, and homozygous animals that are chimeric, heterozygous, or homozygous, respectively, for a modifying polynucleotide integrated into a target polynucleotide. The present invention also includes vectors and methods of using the vectors for integrating a modifying polynucleotide into a target polynucleotide in a cell, for instance, a fish cell or a mouse cell.

 
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< Cell-specific molecule and method for importing DNA into osteoblast nuclei

< Non-inbred embryonic stem cells having good developmental potential

> Novel plant acyltransferases specific for long-chained, multiply unsaturated fatty acids

> Expression cassette encoding a 5-enolpyruvylshikimate-3-phosphate synthase (epsps) and herbicde-tolerant plants containing it

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