The present invention is directed to the field of HIV resistance to RT inhibitors and methods of determining the levels and mechanisms of action of HIV resistance. The methods of the present invention may be accomplished using a novel in vitro assay that provides a reaction well comprising a template for an HIV RT enzyme, a primer, a detectable dNTP substrate, an HIV RT inhibitor, and a ribonucleotide chosen from ATP and GTP or a pyrophosphate. The RT activity is determined by measuring the amount of the detectable dNTP substrate that is incorporated into the template, and the level and/or mechanism of resistance of HIV to the HIV RT inhibitor is determined using the RT activity. The methods may also be used for designing new therapies, screening for new drugs and treatments, and determining the role of mutations in observed resistance.

 
Web www.patentalert.com

< Material and methods for the production of sperm and analysis thereof

< Methods for determining plasma free drug concentration by direct measurement of binding affinity of protease inhibitors to plasma proteins

> Method and kit for detection of tat protein with anti-tat oyi antibodies

> Composition and method for stimulating immune response to pathogen using complex adenoviral vector

~ 00281