An interlocked valve sensor insertion assembly for use in a manufacturing process is shown and described. The assembly includes a ball valve configured to alternately couple and decouple a retraction chamber with the process. An insertion assembly is coupled to the retraction chamber, and includes an axially slidable insertion tube with a sensor supported on one end. The tube has a range of motion capable of extending the sensor into the valve. A fastener releasably couples the tube to the insertion assembly, and an actuator is configured for engagement with both the valve and the fastener. The actuator is configured to alternately engage and disengage the fastener when the valve is respectively disposed in open and closed positions.