MLVA methods for strain discrimination among Mycobacterium tuberculosum strains
are disclosed. Nine VNTR loci have been identified from genomic sequences of Mycobacterium
tuberculosum strains and primer pairs suitable for amplifying the VNTR by PCR
are disclosed. Polymorphisms at these loci were used to resolve genotypes into
distinct groups. This sub-typing scheme is useful for the epidemiological study
of Mycobacterium tuberculosum and may be applied to the local detection
of the pathological causative agent of tuberculosum.