There is disclosed are an enzyme preparation characterized by a polymer carrier having fine pores with average radius of 200-500 and the enzyme is capable of converting an N-substituted cyclic imino ester into an (S)-N-substituted cyclic imino acid, thereby an (S)-N-substituted cyclic imino acid is preferentially produced, and has an amino acid sequence as set forth in SEQ ID NO: 1 or the like.

 
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