The present invention relates to compositions and methods for the detection and
characterization of nucleic acid sequences and variations in nucleic acid sequences.
The present invention relates to methods for forming a nucleic acid cleavage structure
on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific
manner. For example, in some embodiments, a 5 nuclease activity from any
of a variety of enzymes is used to cleave the target-dependent cleavage structure,
thereby indicating the presence of specific nucleic acid sequences or specific
variations thereof.
